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Problem+Festival+_4+total - Problem Festival #4 Patient X...

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Patient X has a history of muscle fatigue, and defective LDH is suspected. A leg muscle biopsy is taken from the patient, and the LDH is purified using the same purification protocol and Shimadzu enzyme assay that we used in our 120L LDH experiment. 1. A portion of the LDH purification table is shown below. Fill in the shaded boxes. Show calculations below the table. Purification sample sample volume dilution for enz assay dA/min total IU total mg protein Specific activity crude extract 5 ml 1/200 -0.021 10.2 dialyzed, purified LDH 0.05 ml 1/4000 52.6 0.065 purified leg LDH from a normal human 0.06 ml 1/1000 -0.052 16.7 0.012 1393 2. Patient X’s LDH does appear to be defective, based on which data above? 3. Next, we want to analyze Patient X’s purified LDH sample using PAGE. Describe how to prepare a 20 μ l sample containing 6 μ g of protein and using 2x sample buffer. 1
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This note was uploaded on 11/21/2010 for the course MCB 120L 69059 taught by Professor Fairclough during the Fall '10 term at UC Davis.

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Problem+Festival+_4+total - Problem Festival #4 Patient X...

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