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Topic%2003%20_%20Kinetics%20_%20F10

Topic%2003%20_%20Kinetics%20_%20F10 - Why study enzyme...

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Why study enzyme kinetics? Compare enzymes from different sources, learn about the evolution of an enzyme, engineer better enzymes, determine how inhibitors affect enzymes, design better drugs that interact with enzymes.
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S P Kinetics How fast? Energetics Which way? G = – RT ln K eq Favorable direction has a G Enzyme Kinetics simple 1 substrate : 1 product
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Energy G P S TS E + enz
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Two ways to measure enzyme activity: 1. Disappearance of S Usually less accurate: hard to detect a small change in [S] when [S] is very high 2. Appearance of P Usually more accurate: starting with [P] = 0, so detecting a small change in [P] is less questionable
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