E XPERIMENT 3: L IPID A NALYSIS- F REQUENTLY A SKED Q UESTIONS Background : A research dietitian designed a series of menus (using food analysis software) for a study with a goal of meeting the DRIs based on a 2,000 kcal diet. A homogenate (i.e., food composite) was prepared and sent to a lab for chemical analysis to measure %lipids, %carbohydrates, %protein, and total energy. What is the purpose? The purpose of this lab is to compare the measured nutrient contents to the expected nutrient contents based on the diet’s design (to meet the DRIs). Why is 400g of dry weight used for the calculations? Once a food composite is blended, it is then desiccated to eliminate moisture (H 2 O). What is left is only dry matter. The dried weight varies depending upon how much food is in the sample. For the purposes of this lab, we assume a 2,000 kcal diet, which yields approximately 400 g of dry matter after desiccation. This dried sample can then be analyzed chemically to determine nutrient contents. What is Ash?
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