Lecture 22 Notes - Bio 93 Fall 2009 Lecture 22 Recombinant...

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Reading: Chapter 20, pages 396-423 Bio 93 Fall 2009 Lecture 22 Recombinant DNA technology Monday 11/18/09
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The function of enhancers is an example of Transcriptional control of gene expression
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How is the word “clone” defined? 1) A lineage of genetically identical individuals or cells 2) A single individual organism that is genetically identical to another individual 3) To make one or more genetic replicas of an individual or cell What does it mean to clone a gene? The production of multiple copies of a gene
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How do you clone a gene?
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How do you make a recombinant DNA molecule? Restriction enzymes normally protect bacteria by cutting up foreign DNA from other organisms or phages
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Cloning genes into bacterial plasmids:
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Amplifying DNA in Vitro : The Polymerase Chain Reaction (PCR) Method of generating large quantities of a particular gene or sequence of DNA Uses primers that bracket the desired sequence Uses a heat-resistant DNA polymerase Can work with as little as one molecule of DNA
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