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2 buffer - Titration of a buffer Purpose To find the pKa(s...

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Titration of a buffer Purpose: To find the pKa(s) value of a buffer using an acid base titration Ecuation Henderson-Hasselback Introduction What are acids and bases? Define buffers and give examples of buffers and their uses in biology Explain how to prepare buffers and how to select them Derive the Henderson –Hasselback equation from an acid dissociation and indicate its uses Write structural formulae and pKa of at least 5 different buffers used in biology. Questions Ch2: # 21, 23, 25, 27 and 29 Safety and waste MSDS: NaOH and HCl Dispose all aqueous solutions in the sink. Consumables Kimwipes Select one standard buffer from the front cart Dispense standard buffers for calibration (pHs 4, 7 and 10) Common usage Magnetic stirrer (make sure the heat is OFF) Buret pH meter and pH probe General considerations Wash the buret at the beginning and end of the experiment. All plots for this and future experiments, should be done using a computer program. Hand made plots are not accepted. Plots should have a descriptive title (different than the units of the axes), axis should be labeled and data points should be included on the side. Initial and date each plot.
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