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Unformatted text preview: Buret pH meter General considerations This titration requires the same set up used for buffer titration. The differences are a) a solution of aminoacid will be provided instead of solid b) the NaOH and HCl used for the titration are 0.2 M instead of 1.0M c) You will require to use both acid and base for the titration, make two- 10 mL aliquots; one for acid titration and one for base. Change the buret with a team that is using acid/base to perform the second titration. Procedure 1) Calibrate the pH meter 2) Using a volumetric pipette transfer 10 ml of unknown amino acid solution to a 150 mL beaker. Add approximately 50 ml of water. 3) Titrate the aminoacid with acid or base then change buret and solution to do the second titration. 3) Plot the data as in buffer titration 4) From the amino acids given: Identify the aminoacid and determine the concentration of the unknown solution....
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This note was uploaded on 11/19/2010 for the course BIO 360 taught by Professor Fink during the Spring '10 term at UCLA.
- Spring '10