5 agarose

5 agarose - Agarose Gel Electrophoresis Purpose: To find...

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– Agarose Gel Electrophoresis Purpose : To find the size of fragments of DNA using 0.7 % agarose gel electrophoresis Chem formula Ethidium bromide , agarose Introduction: Define electrophoresis, what is it used for, what is the principle of the separation, common media for the gel matrix, visualization agents write formula when applicable, how do they bind to DNA, Describe how to obtain the molecular weight of an unknown Describe agarosa gel electrophoresis and compare to SDS-PAGE Questions Ch 5 # 29, 30, 32,37. Safety Ethidium bromide Consumables Kimwipes Pipette tips Common usage Electrophoresis chamber with comb and power supply Micropipettes Hot plate stirrer Uv-box and lamp general considerations The separation will be performed using a mini Single Cell Electrophoresis chamber and comb with 8 or 10 wells Procedure: Day 1 a) Lift and secure the sides of the gel caster tray to form a tray it on the.
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5 agarose - Agarose Gel Electrophoresis Purpose: To find...

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