Ch10 replication-1

Ch10 replication-1 - Replication of DNA Naturally occurring...

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Replication of DNA • Naturally occurring DNA exists in single-stranded and double-stranded forms, both of which can exist in linear and circular forms • Difficult to generalize about all cases of DNA replication • most of the details were first investigated in prokaryotes, particularly E. coli
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Flow of Genetic Information in the Cell • Mechanisms by which information is transferred in the cell is based on “Central Dogma”
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Prokaryotic Replication • Challenges in duplication of circular double-stranded DNA • achievement of continuous unwinding and separation of the two DNA strands • protection of unwound portions from attack by nucleases that attack single-stranded DNA • synthesis of the DNA template from one 5’ -> 3’ strand and one 3’ -> 5’ strand • efficient protection from errors in replication
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Separation of the two original strands and synthesis of two new daughter strands using the original strands as templates
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Which Direction does Replication go?
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DNA Polymerase
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• The 3’-OH group at the end of the growing DNA chain acts as a nucleophile. • The phosphorus adjacent to the sugar is attacked,
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Ch10 replication-1 - Replication of DNA Naturally occurring...

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