STAT530lecture3IntroMicroarrayBW - STAT 530 Introduction to...

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Ping Ma STAT 530 1 STAT 530 Introduction to Microarray Ping Ma 2 Ping Ma STAT530 Microarray s High throughput gene expression platforms – SAGE – Microarrays: cDNA and oligonucleotide arrays s Microarray analysis: – Very low level: image analysis – Low level: normalization • Dye swap • Linear scaling • Loess: reference set • More next lecture…
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Ping Ma STAT 530 2 3 Ping Ma STAT530 Central Dogma of Molecular Biology DNA RNA Protein Physiology DNA replication Transcription Translation Folded with function Reverse transcription 4 Ping Ma STAT530 Differential Expression s Understand expression level of genes under different conditions – Cell types (brain vs. liver) – Developmental (fetal vs. adult) – Response to stimulus (rich vs poor media) – Gene activity (wild vs. mutant) – Disease states (healthy vs. diseased)
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Ping Ma STAT 530 3 5 Ping Ma STAT530 High Throughput Gene Expression Technologies s Measure gene expression: quasi-estimate of the protein level and cell state s High throughput: measure mRNA level of every gene in the genome s Formats: – Serial Analysis of Gene Expression (SAGE) – Microarrays: spotted and cDNA microarrays oligonucleotide arrays 6 Ping Ma STAT530 SAGE s Serial Analysis of Gene Expression s Earliest high throughput gene expression experiment s Velculescu et al, Science 1995.
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4 7 Ping Ma STAT530 SAGE data 8 Ping Ma STAT530 SAGE data s A SAGE experiment: ---sampling a handful of colored balls from a
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STAT530lecture3IntroMicroarrayBW - STAT 530 Introduction to...

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