lab - The Effects of Temperature and pH on the Reaction of...

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The Effects of Temperature and pH on the Reaction of Amylase with Starch Ashley Serber 9/21/07 TA: Joysee Rodriguez BSC2010L Sect. 0541
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Abstract This experiment was to find the optimum temperature and pH for a starch/amylase reaction. The optimum temperature of the reaction is the temperature at which the reaction rate is the highest and the optimum pH is the pH at which the reaction rate is the highest. If the temperature is too low the reaction will slow greatly and if the temperature is too high it will damage the enzyme. If the pH is too low or to high it will also damage the enzyme slowing the rate greatly. I found theses results by talking the starch and amylase mixture and placing it in a spectrometer to find the absorbance. I repeated this several time at different temperature and pHs and checked the absorbance over time (20 min). From the experiment I found the optimum temperature to be 60 degrees and optimum pH to be 5.5. From these results I concluded that the temperature has to be fairly high and the pH has to be more acidic. Introduction The experiment was important because we are seeing what the optimum temperature and pH is for the reaction of the enzyme Amylase. Enzymes are the catalyst in a biochemical reaction. They help speed up the reaction with out changing the reaction. Enzymes, like amylase, help make a reaction more efficient. The do this by lowering the activation energy required by the reaction. The substance that acts with the enzyme is called the substrate. In the experiment the substrate was starch. The area where the enzyme and substrate meet is called the enzyme- substrate complex. This eventually breaks down in to the product and the enzyme. There are two factors that determine the rate at which the reaction occurs; the time it takes the enzyme to effect
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the substrate and the frequency of the two joining. Factors within the environment can effect the rate of reaction also, such as temperature and pH. When a reaction is heated, the heat speeds up the molecular motion; it increases the
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This note was uploaded on 04/03/2008 for the course BSC 2010 taught by Professor Bowes during the Spring '08 term at University of Florida.

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lab - The Effects of Temperature and pH on the Reaction of...

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