Prokaryotic Gene Regulation with keys

Prokaryotic Gene Regulation with keys - Prokaryotic Gene...

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Prokaryotic Gene Regulation Lac Operon A operon is the entire stretch of DNA that includes the operator, the promoter, and the genes that they control Operon on-Gene expression, Operon off-No gene expression 1) When is lacI expressed? What protein does it encode? lac Repressor gene ( lac I ) or I makes repressor protein own promoter and terminator 2) What proteins do lacZ, lacY, and lacA encode? lac Z – encodes b- galactosidase lac Y – encodes permease lac A – encodes an enzyme that adds an acetyl (CH 3 CO) group to lactose
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3) For each combination of sugars below, determine if the repressor is active, if the levels of cAMP are high or low, if CAP is active, and if lac operon expression will occur. (Use the activity and flow chart on the next page) a. Glucose only b. Lactose only c. Fructose only d. Glucose and Lactose e. Glucose and Fructose f. Lactose and Fructose Condition Negative regulation Positive regulation Final result Lactose and Glucose Allolactose (inducer) high
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This note was uploaded on 11/29/2010 for the course BIO 311C taught by Professor Satasivian during the Fall '08 term at University of Texas at Austin.

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Prokaryotic Gene Regulation with keys - Prokaryotic Gene...

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