TRANSCRIPTION and TRANSLATION WORKSHEET[1] WITH KEY

TRANSCRIPTION and TRANSLATION WORKSHEET[1] WITH KEY -...

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TRANSCRIPTION WORKSHEET SPR10 1) Consider the following eukaryotic pre-mRNA molecule. The underlined sections are introns. 5’-AUCGGUUACCGCCAUGCCAGAUUAU UAAACGCGA GGCGGC CGGCCG UU AUAUGCUAACU AAUAAA UCCG-3’ a) What alterations it needs to go through to become a mature mRNA? Splicing and addition of 5’ cap and poly A tail in 3’ b) What is the role of AAUAAA ? Termination of transcription and signal for poly A tail 2) Process Starting molecule (template) New molecule synthesized Polymerization or processing enzyme Replication DNA DNA DNA polymerase 1. Transcription DNA mRNA RNA polymerase 2. RNA processing Pre-mRNA Mature mRNA Spliceosome 3(a) In prokaryotes, replication and transcription occurs in which part of the cell? ___ Cytoplasm ___________ (b) In Eukaryotes, replication and transcription occurs in which part of the cell? ____ Nucleus ___________ 4) For the following ds DNA, the bottom strand is the template strand:
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5’-C T A G G G A A T C G G C A T-3’ 3’- G A T C C C
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TRANSCRIPTION and TRANSLATION WORKSHEET[1] WITH KEY -...

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