Bio+97+Worksheet+8 - Bio 97 Worksheet 8 1. Phage display 2....

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Bio 97 Worksheet 8 1. Phage display A. DNA fragments reverse-transcribed from genomic mRNA found in the nucleus of an organism & then inserted into a collection of host cells; together these constitute the genome of the organism 2. In situ hybridization 3. cDNA library 4. DNA library B. Genomic DNA which has been digested by a restriction enzyme for different lengths of time, separated by size using agarose electrophoresis, then isolated & ligated into vectors which are taken up by host cells (aka “shotgun sequencing”) 5. Restriction enzyme C. An enzyme that cuts double-stranded or single stranded DNA at specific recognition nucleotide sequences known as restriction sites D. Uses a labeled complementary DNA or RNA probe to localize a specific DNA or RNA sequence in a portion or section of tissue E. High-through-put method to elucidate protein-protein, protein-peptide, and protein-DNA interactions that uses bacteriophages to connect proteins with the genetic information that encodes them
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Bio+97+Worksheet+8 - Bio 97 Worksheet 8 1. Phage display 2....

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