Bio+97+Worksheet+Worksheet+7+Key - Bio 97 Worksheet#7...

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Bio 97 Worksheet #7 Conceptual problems : Conjugation , Transformation , Transduction 1) Why is generalized transduction not the best method for initially mapping 2 formerly unknown mutants relative to one another on the bacterial chromosome? Answer: Because it is unlikely that 2 genes drawn at random would be so closely linked that they would be co-transduced. Co-transduction is a minimal requirement of mapping genes by this method. If they are too far apart, the linkage in the chromosome is automatically broken, and therefore, unmeasureable during the fragmentation of bacterial DNA that accompanies DNA packaging by transducing phages. 2) How does transformation with fragments of the bacterial chromosome differ from transformation with plasmids in the fate of the donor DNA? Answer: In transformation, fragments of bacterial DNA must become part of the recipient’s chromosome in order to become permanent – that is, to replicate. Plasmids, on the other hand, can replicate without integration, making transformation more efficient once they enter cells.
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