AnswerKey_Worksheet_1 - Bio 97 Genetics Dscussion...

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r.) Dl.{Apolymerization occurs in what directionP 5 '- 3' , 2.) TheDNAtemplate is read in what directionP 3t-) V) s.) DN{Ais said to have polarity. Describe why an$ also the g{oups that you would expect to find at each end of a single straniofDNA . 6t ."end -a eho ;1vl^ft !"-nV "--: _ "'"^-; et enJ. 4 hy){ruxyl !"r'}. (now lvte efldt qrc d,itfeQrrl ! ' +a) hio hliqvrt"d thtn r' '/ 'pQk+S I i 4,.) You were recently hired to work at a biotech company and your PI is studying the effects ofDrug 614 ph il: f" y1p 1 p Xon gene regulation. You are asked to exhact total RNA from rat tissue, convert it all into cDNA, yOt t fgWre vvr76 {- and perform PCRin order to determine which genes are up/down regulated. On the axis below, h1trlw tgfnp 1 draw out a diagram (graph) showing each PCRtemperature and label the reaction. Describe e hftnq €,f .' eachreaction. Remember,thatforeachreaction,thetemperatureisheldforsometime. [email protected]' frt cycle---j ?CR? usuc{ttv Temp('c) l#1...-...t1-t+n(,\1 r.emp("c) t K- *ocyOlu X don+ wrwt r I @-*-a 'r.
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