Eleanor Levine - controls to verify any conclusions drawn...

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Eleanor Levine 11-6-10 Bio 210-1, Lab Section 73 Lab Partner: Abby Peterson Lab Report 4: Bacterial Gene Transfer 1. The purpose of using just CSH121 and CSH125 separately as controls is to be sure that any growth is actually due to the mutual presence of an F(-) strains and F(+) strains in the same media. The CSH121 is the Hfr strain, the donor, and has a high frequency of recombination. The CSH125 is the F(-) strain and is the recipient which lacks a fertility factor. When each are alone on a medium, it is expected that no growth could occur because a growth requires the presence of a donor and a recipient. Although this is expected, the experiment must test them as
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Unformatted text preview: controls to verify any conclusions drawn from the data. 2. It is important to make dilutions in Part 1 to obtain single colonies because these strains of cells will be able to transfer at a high rate. In order for the process of conjunction to occur effectively, it must be between two single cells, a donor and a recipient. A pore forms solely between those two and transfers DNA copies to the F(-) cell and the transfer is only complete when the whole copy was transferred to it. Diluting the samples so that we obtain only single colonies allows this process to occur more rapidly and effectively....
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