Ch_12_Bio_Study_Guide-katie - Ch 12 Bio Study Guide...

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Ch 12 Bio Study Guide -Transposons “jumping genes” were the first example of mobile DNA elements McClintock used the changes in the corn kernel were explained by movement of DNA elements in chromosome (Fig 12.1) Bacteria- mobile elements critical for evolution and distribution of new functions that benefit the organism (antibiotic resistance) -Three ways to move mobile genetic elements 1. transposition and retroviral integration 2. conservative site-specific recombination 3. target-primed reverse transcription 1. transposition - process when DNA entity can move between DNA sites that lack homology using a self-encoded recombination protein called transposase. -DNA transposases- move as a DNA element -LTR (long terminal repeat) retrotransposases- move with RNA intermediate almost identical to the movement of retroviruses, common in eukaryotes, rare in bact -IS elements 2. CSSR- segment of DNA moves between specific recombination sites as DNA using a cognate recombinase 3.TPRT- a free 3’ end of broken target DNA is used to prime a replication process, which allows a DNA copy to be made of an element that moves as a segment of DNA Transposition - DDE – indicates the active site of the transposase Insertion sequences - simplest transposable elements in bacteria Anatomy of a transposon - transposase gene is between inverted repeats. Transposase binds inverted repeats and excises transposon. May be many transposase binding sites,
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Ch_12_Bio_Study_Guide-katie - Ch 12 Bio Study Guide...

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