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Quiz 1 – HistologyOrganelles -For each organelle know function, structure, appearance on LM and EM -Understand how cellular function and subcellular components are related (i.e depending what organelles are in a cell what could be the function of the cell?)Organelle FunctionAppearance Relation b/w Cell Function & OrganelleNucleus Contains genetic material as heterochromatin (tightly bound = more e- dense = darker) & euchromatin (loosely bound = less e- dense = lighter) Bidirectional RNA & protein transport through nuclear poreNucleus appears as purple/blue on LM -Only organelle consistently seen, the nucleolus will be darker -Heterochromatin =darker H&E = more basophilic, EM = more e- dense -Euchromatin = lighter, H&E = less basophilic, EM = less e- dense RERSynthesis of secreted proteins (on ribosomes) & membrane proteins – continuous with nuclear membrane Rough = ribosomes H&E stains blue b/c basophilic (-) charge from the ribosomes + RNAEM – series of flattened membranes dotted with dark grey ribosomes Cell with a large RER = a cell that produces a lot of proteins Ex. exocrine pancreatic cellsSER Synthesis of cell membrane components, steroid hormones,detox enzymes, Continuous with RER but no ribosomes (smooth) – flattened/tubular sac-likeCells with prominent SER roles: detoxification (detoxification enzymes located in SER)ex liver cells, production of hormones ex. adrenal gland
glycogen metabolism, sequestration andrelease of CaEM – appears as whiteblobs LM – pink net like structure (eosinophilicb/c of (+) proteins) Golgi CIS Golgi closest to RERTRANS Golgi furthest from RERMaterial enters cisand exits trans Glycosylation of membrane proteins & proteinsorting Stack of flattened membrane sacks LM – appears as clear area in the cell EM – Flattened stackCells with prominent Golgi are specialized to produce a lot of proteins (ex. plasma cells – produce antibodies (protein)