4147497-Experimental design

4147497-Experimental design - 4147497 Experimental design...

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4147497 Experimental design () Forty eight Ross 308 broilers (50% male and 50 %female), weighing approximately 2.2 kg and previously fed one of six experimental diets (negative control (NC) based on a maize/soy diet, positive control (PC) NC + 250 international units phytase (FTU), NC +500 FTU, NC +2500 (FTU) + 37500 FTU) were used. Birds were check for health conditions and approved. Birds were randomly choose for this experiment from the previous experiments and individually allocated in separate cages and fed with ( C ) finisher diet in a randomised block design. hypothesis can be modified The birds were previously fed phytases in the diet; The hypothesis of this experiment will be whether the birds will respond the same way as they were feed phytase previously .or see if there will be a carry over affect of phytase activity in pertaining to performance if they are being feed (NC )finisher diet maize-soybean based diet prior there five diets. An adapted ad libitum feeding technique was conducted to avoid any problems due to feed intake and allow the availability at all times. The aim of the experiment was to compare the effect of the previously fed dietary phytase activities on Gross energy (GE), Nitrogen, dry mater of excreta and feed, phytate phosphorus, feed intake and feed conversion ratio. Water was supplied ad libitum throughout the study via a suspended nipple drinker line. placed in individual cages (0.5 m x 0.8 m floor area) designed for excreta collection, at a constant house temperature of C ° 20 and 23 hours of light per day. Excreta were quantitatively collected after 48 h after ad libitum feeding excreta was, dried and weight. The broilers was weight and feed was weight before and after placing in individual cages to determine feed conversion ratio, performance etc Data and results are attached • Broiler Performance (feed intake, weight gain, feed conversion ratio) The results should be run in statistically program ANOVA Topic Description : ================= (some added info)Introduction Phytases (myo-inositol hexaphosphate phosphohydrolases) are a specific class of phosphatases that catalyse the sequential hydrolysis of phytate to less phosphorilated myo-inositol derivates and increase the availability of dietary minerals and nutrients (Irving and Cosgrove, 1974; Wyss et al., 1999; Oh et al., 2004). Although endogenous phytase is determined in the gut of poultry (Maenz and Classen, 1998; Applegate et al., 2003), its efficacy and amount produced in the gastrointestinal tract
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(GIT) is insufficient to hydrolyse phytate structure and to remove the negative effects possessed by dietary phytates . Exogenous phytases were widely used in the poultry industry for the last decades to improve the utilisation of dietary minerals, especially phosphorus, dietary energy and amino acids, bird performance and reduce environmental pollution (Zyla et al., 1994; Waldroup, 1999; Selle et al., 2000; Rutherfurd et al., 2002; Cowieson et al., 2006). Despite the fact that lots of research was done on phytases, their mode of action is still not well understood. In general,
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