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Lab 2 - Patterns of Inheritance_new

Lab 2 - Patterns of Inheritance_new - Laboratory 2 Patterns...

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Laboratory 2 Patterns of Inheritance ►►Bring a calculator to lab Before the lab Print and read all lab documents and remember to bring these to lab including the additional readings Complete the PRELAB worksheet. Objectives 1. Understand the meaning and connections between the following terms: a. Phenotype and genotype b. Locus, gene and allele c. Homozygous and heterozygous d. Dominant and recessive e. Genetic variation 2. Consider both the pattern of inheritance and the possible evolutionary significance of certain human traits. Timeline 2:10 – 2:20 Brief Introduction 2:20 – 2:40 Pedigree Case Study Discussion 2:40 – 2:50 Colour Sensitivity Exercise 2:50 – 3:50 PTC bitter taste sensitivity 3:50 – 4:40 INLAB Case Study 4:40 – end Clean up Introduction Though Mendel and others asked the question of why offspring resemble their parents, the reverse question is also important—why do even closely related individuals vary considerably in appearance and behaviour? These differences exist because all individuals inherit unique combinations of genes from their parents. Unique combinations of genes are a direct result of meiosis acting on the shuffling of different alleles. In this lab we will look at some human traits to study Mendelian genetic patterns of inheritance. Materials Hand lens or illuminated magnifier Phenotype charts Colour charts Series of dilutions of PTC with Q-tips Part 1 – Pedigree Case Study Discussion (to be lead by your TA) Part 2 - Red-green colour vision ( Read the additional information in Appendix A) Red-green ‘colour blindness’ is inherited as an X-linked recessive trait. During lab you will be screened for this trait. BIO152 2010 University of Toronto at Mississauga
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5-Patterns of Inheritance - 2 of 9 Your TA will carry out the experiment on one member of your group. This person will then complete the experiment for each group member. Designate one member from your bench to test the others at your bench. Part 3 - PTC tasting Read the additional information about PTC posted on the separate site: http://learn.genetics.utah.edu/units/basics/ptc/ The inability to taste phenylthiocarbamide (P.T.C.) or phenylthiourea is conditioned by a recessive allele (t). The majority of the North American Caucasian population are ‘tasters’ (T), experiencing a striking bitter or sour sensation when this substance is put on the tongue. Children of two non-tasters have non-taster children. However, expression of the allele is variable. 1. Count your taste buds (fungiform papillae) Accessed August 12, 2006 and reprinted from http://www.bbc.co.uk/science/humanbody/body/articles/senses/supertaster.shtml Conduct a simple scientific experiment to find out whether you have a supertaster tongue or not. Blue food colouring Cotton buds Reinforcement rings Magnifying glass Figure 1 Comparison of the number of papillae in various types of tasters Procedure 1. Using a cotton Q tip, swab some blue food colouring on to the tip of your tongue 2. Place a reinforcement ring on your tongue 3.
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