Lab 4-Evolution and Bacteria

Lab 4-Evolution and Bacteria - BIO 152 - Laboratory 4 -...

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BIO 152 - Laboratory 4 - Real time evolution in bacteria Natural Selection in action—Evolution as we speak… ► Bring a calculator to lab Before this lab 1. Read this lab description carefully including the Appendix; print a copy to bring to lab. 2. Review the Lab Safety Guidelines available on Blackboard. 3. Complete PRELAB Worksheet before lab. 4. Print and bring to class INLAB Worksheet. 5. Don’t forget to bring your lab coat to lab. Objectives by the end of this exercise you should be able to: 1. List and describe safe lab practices to work with bacteria. 2. Discuss the relative effectiveness of antiseptics, disinfectants and antibiotics to control bacterial growth. 3. Discuss how natural selection affects bacteria populations’ sensitivity and resistance to antimicrobial agents. 4. Distinguish between a hypothesis, null hypothesis, and alternative hypothesis. 5. Formulate predictions from a hypothesis. 6. Describe the component steps of the scientific method (observation, hypothesis, prediction, experimentation, and conclusion) and identify these steps from a description of an experiment. 1) Antimicrobials and E. coli bacteria sensitivity 1. resistance versus sensitivity 2. antimicrobial—antibiotic, disinfectant, antiseptic, spice 3. bacteri cidal versus bacterio static 4. bacteria-- prokaryote 5. clonal reproduction 6. horizontal gene transfer 7. pathogen 8. sterilization 9. zone of inhibition 2) The scientific process and experimental design 1. data 2. evidence 3. experimental method (procedure for experiment also called protocol) 4. scientific method 5. hypothesis (null hypothesis, alternative hypothesis) and prediction 6. control 7. observation 8. replication 9. variables: dependent, independent, standardized BIO152H5F 2010 University of Toronto at Mississauga
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Real time evolution 4 - 2 Lab Timeline: 2:10 – 2:20 (10 minutes) Introduction 2:20 – 3:20 (60 minutes) Exercise 1 Setup plates for bacteria experiment Clean up your work area before proceeding to the other exercises 3:20 – 4:20 (60 minutes) Exercise 2 Simulated bacterial resistance 4:20 – 4:50 (30 minutes) Exercise 3 Protecting ourselves from food 4:55 – 5:00 (5 minutes) Final clean up—marks deducted from team leaving without cleaning up! Additional background information The Howard Hughes Medical Institute (HHMI) site is great for many things including “Antibiotics Attack” for another description about antibiotics: You also might look at the comparison of antibacterial and regular soap from HowStuffWorks Background Bacteria are found almost everywhere. While most species are beneficial, some are harmful or even pathogenic (cause disease). Chemical and physical agents may be used to control bacterial growth; however, when these agents are used inappropriately bacterial species can become resistant, by the process of natural selection. In this exercise you will learn more about the genetic diversity of bacteria
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This note was uploaded on 11/30/2010 for the course BIO BIO152 taught by Professor Cordon during the Fall '09 term at University of Toronto- Toronto.

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Lab 4-Evolution and Bacteria - BIO 152 - Laboratory 4 -...

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