3AL Lecture 6 - Chromatography Separation of Different...

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Chromatography Partition Adsorption Separation of Different Compounds
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stationary phase (side view) adsorb compounds onto stationary phase mobile phase Adsorption Chromatography Compounds that have Different Interactions with the Mobile Phase Versus the Stationary Phase can be Separated Compound C Stronger interactions with the mobile phase and/or weak interactions with the stationary phase leads to more time moving with the mobile phase. Compound A Stronger interactions with the stationary phase leads to less time moving with the mobile phase.
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Stationary Phases Polar Non-Polar Ionic silica gel alumina reverse phase cation exchange anion exchange
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Adsorption onto Silica Gel
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1-Butanol and a Non-Polar Mobile Phase 1-butanol adsorbed onto silica gel
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1-Butanol and a Polar Mobile Phase 1-butanol adsorbed onto silica gel
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Thin Layer Chromatography (TLC) Stationary Phase is Applied to a “Backing” as a Thin Layer Backing Glass Aluminum Plastic
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TLC: The Technique Step 1: Preparing the Developing Chamber Add mobile phase Add flter paper Allow time For saturation Cover the chamber
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3AL Lecture 6 - Chromatography Separation of Different...

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