3AL Lecture 7 - Retention Factor (Rf) Not a Physical...

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R f = distance to midpoint of spot distance to solvent front Retention Factor (R f ) Not a Physical Constant R f depends on: The stationary phase The mobile phase The temperature The amount of compound spotted
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TLC: Conclusions Given: Same stationary phase and same mobile phase If R f values from two plates are very different: then the compounds are different If R f values from two plates are similar or the same but the visualization data is different: then the compounds are different If R f values from two plates are similar or the same and the visualization data is the same: then the compounds might be the same or different
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TLC: Conclusions R f values have inherent errors Accuracy of the solvent front Measurement to the midpoint of the spot Amount of compound applied to the TLC plate
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TLC: Conclusions Co-spotting spot the two compounds on top of each other spot the two compounds next to each other spot the two compounds on top of each other are not the same might be the same might be the same
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TLC: Conclusions TLC Cannot be used to Defnitively Establish the Identity oF a Compound
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TLC: Conclusions However: If it walks like a And it talks like a Then it’s probably a
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3AL Lecture 7 - Retention Factor (Rf) Not a Physical...

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