10 - http/www4.ncsu.edu/~knopp/BCH451/e02a3.htm BCH 451...

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BCH 451 Spring 2002 Exam #3 F = 96.48 kJ/mol-V; k = 1.381 x 10 -23 J/K; N o = 6.022 x 10 23 /mol; R = 8.315 J/mol-K 1. Draw the predominate structure of the following molecules at the pH indicated. Be sure to include all carbon and hydrogen atoms! Some constants: a. (4) GpppTp at pH = 7.5 in the syn conformation b. (3) 1-palmitoleoyl-2-linolenoyl phosphatidyl inositol 2. (1) PUZZLER If the dinucleotide in question 1 were cleaved from one end of mRNA from eukaryotes, state which end would it come from and circle the position(s) of any modification(s) on the structure which you have drawn. 3. (1) Draw the structure of cAMP at pH = 6.5 4. For mRNA, a. (0.3) give the full name: b. (0.5) give the size or size range in # of bases: c. (2) give a complete description of its function within the cell: 5. (1.4) Based on the following dideoxy sequencing gel, give the sequence of the DNA template: [use the blanks below for your answer] 6. (2) In the following structure, circle and name each bond which can be hydrolyzed. For each of these bonds, indicate
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