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20 - transducer 5 Circle the RNA type which is specified on...

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BCH 451 Fall 2006 Exam #3 NAME 1. Draw the predominate structures of the following molecules at the pH indicated. Be sure to include all carbon and hydrogen atoms! a. (1.5) A = cAMP; B = IP 3 [pH = 7] b. (4) L = lactose; S = sucrose @ pH = 7 [You can “buy” the biochemical name of your structure for 2 points.] c. (4) C = 1-oleoyl-2-linoleoyl-phosphatidyl-choline; D = 1-linolenoyl-2-steroyl-phosphatidyl-ethanolamine @ pH = 8 d. (4) H = pdUppddG; J = pCpppdA @ pH = 7 [draw purines in anti and pyrimidines in syn ] 2. (1) What is the biological function of restriction enzymes? 3. (2) PUZZLER Why are the sequences which are recognized by restriction enzymes palindromes? Will we find any restriction enzymes with recognition sequences which are not palindromes? 4. (4) Give one pro and one con argument for RAS being a transducer. [Hint: you might start by describing a
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Unformatted text preview: transducer.] 5. Circle the RNA type which is specified on the first page: M R SN T a. (0.3) give the full and complete name: b. (0.7) give the size(s) or size range of this type in units of bases: c. (2) give the full description of the function of this type within the cell: 6. (1 for each) What are the advantages of polysaccharides over monosaccharides for storage of glucose? [Use full sentences.] 7. (4) Describe the Sanger’s dideoxy sequencing technique and how one will determine the sequence of the template: 8. (2) Explain [describe] how stacking interactions stabilize double helices: 9. (4) Why does Dr. Knopp say that melting is a thermodynamic process and annealing is a kinetic process? http://www4.ncsu.edu/~knopp/BCH451/e06c3.htm 1 of 1 4/27/2010 4:14 PM...
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