Midterm II Fall 09 - BIS101-01 Fall 2009 Dr D J...

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BIS101-01, Fall 2009 Dr. D. J. Kliebenstein, Instructor Midterm Exam II Please print your name and ID number on page 2 and the rest of the exam. Do not print your ID number on the first page. The exam has 10 questions and a total of 120 points For full credit, show all of your work. AUTHORIZATION FOR PUBLIC DISTRIBUTION OF GRADED PAPERS OR EXAMINATIONS I,_________________________________, Authorize the University to publicly distribute my graded exam (e.g., handed out in class or left in an exam bin) for BIS101-01. If I do not sign, then I must see the instructor to pick up my exam. Signed:_______________________________________ Dated:__________________________________
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Name:_________________________ ID:__________________________ 1. ____ ___ 2. ____ ___ 3. ____ ___ 4. ____ ___ 5. ____ ___ 6. ____ ___ 7. ____ ___ 8. ____ ___ 9. ____ ___ 10. ____ ___ Total ________
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Name:_________________________ ID:__________________________ 1. (18 points) Complete the following Table, assuming that the DNA is written with the top left being a 5’ end and that the columns represent transcriptional and translational alignments. Further, reading is from left to right. For DNA, use ACTG or N where N represents any possible nucleotide. Notice the questions to the right of the codon table. A A DNA Double Helix A G G T U mRNA G tRNA Anticodon MET SER Amino Acids Which of the above rows is the coding strand?
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Name:_________________________ ID:__________________________ The bottom table is typically thought of as describing the tRNA’s, are there any codons that do not correspond to tRNA’s? If so, what are they and what do they do? 2. We discussed two types of donor DNA that can be used to make libraries to find the DNA for a gene. (8 pts) a. What are these two types of DNA and where did they come from? b. Which type of library would you use for functional complementation when studying a prokaryote? c. Which type of library would you use for functional complementation when studying a eukaryotic gene?
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