Online source - Lab 1: Osmosis and Diffusion I. Objectives:...

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Sheet1 Page 1 Lab 1: Osmosis and Diffusion I. Objectives: · Demonstrate diffusion across a semi-permeable membrane · Measure the effects of various concentrations of solute in the process of osmosis · Differentiate between hypotonic, isotonic, and hypertonic environments · Measure and calculate water potential · Examine the effects of osmosis on plant cells II. Background: Diffusion: The spontaneous movement of molecules or particles in solution along a concentration gradient (i.e. from areas of high concentration to a low concentration) until there is an equilibrium. Osmosis: The diffusion of water molecules through a selectively permeable membrane from a region of low solute concentration to a region of high solute concentration. Equilibrium: A condition in which all influences acting upon it are canceled by others, resulting in a stable, balanced, or unchanging system. Selectively Permeable Membrane: A membrane that allows only certain materials to pass through it by diffusion. Hypotonic: A condition in which the inside of the animal or plant cell has a higher solute concentration than its environment. Osmosis causes a net flow of water into the cell, causing swelling and expansion. This swelling may cause cells without a rigid cell wall to burst. Hypertonic: A condition in which the environment has a higher solute concentration than inside the animal or plant cell. Osmosis pressure causes water to flow out of the cells and causing the cell to shrink. Isotonic: A solution of equal solute concentration that has no net flow of water across the selectively permeable membrane. Water Potential: The physical property predicting the direction in which water will flow, governed by solute concentration and applied pressure f water to move. Osmotic Potential: The potential for water to move across a selectively permeable membrane, where the osmotic potential of pure water is 0 and any water movement is measured with a negative value. (Also known as solute potential) Pressure Potential: The physical pressure on a solution Osmotic Pressure: The pressure that must be exerted on a solution containing a given concentration of solute separated from a sample of the pure solvent by a membrane.
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Sheet1 Page 2 III. Data: A. Table 1: Diffusion Color Glucose Content Time Start 30 minutes Dialysis Bag cloudy, white light blue near the top, cloudy white at the bottom Beaker yellow brown layer of yellow/orange cloudy Dialysis Bag Beaker Yes No Yes Yes B. Table 2: Solution Water 0.2 M 0.4 M 0.6 M 0.8 M 1.0 M Osmosis Investigation Dialysis Bag Initial Mass (g)
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Sheet1 Page 3 16.99g 25.13g 25.48g 13.68g 27.26g 23.25g Dialysis Bag Final Mass (g) 18.50g 25.69g 27.46g 14.27g 30.12g 25.92g Change in % Change in Mass (g) Mass +1.51g +8.89% +0.56g +2.23% +1.98g +7.77% +0.59g +4.31% +2.86g +10.49% +2.67g +11.48% + Osmosis C. Table 3: Potato Cell Water Potential
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Online source - Lab 1: Osmosis and Diffusion I. Objectives:...

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