HAU16008-02-2 - The Mechanism of Plant Transformation...

Info icon This preview shows pages 1–4. Sign up to view the full content.

View Full Document Right Arrow Icon
1 The Mechanism of Plant Transformation Lecture 2 October 28, 2008 Core Concepts : Broad host range plasmid Chimeric genes Selectable marker genes Scorable marker genes Disarmed Agrobacterium strains Cointegrating vectors Binary vectors Multiplication/amplification of DNA molecules. Plasmid vectors -Narrow host range vectors -Broad host range vectors Cloning and Genetic selection -Cloning DNA fragments -Transformation of bacteria -Antibiotic selection Plasmids are autonomous replicating units capable of replicating in a limited (narrow host range) number of bacteria or broad (broad host range) range of different gram -ve bacteria by virtue of their replication regions. They also have well defined maps and many single restriction enzyme sites
Image of page 1

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full Document Right Arrow Icon