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1The Mechanism of Plant TransformationLecture 2October 28, 2008Core Concepts:Broad host range plasmidChimeric genesSelectable marker genesScorable marker genesDisarmed AgrobacteriumstrainsCointegrating vectorsBinary vectorsMultiplication/amplification of DNA molecules.Plasmid vectors-Narrow host range vectors-Broad host range vectorsCloning and Genetic selection-Cloning DNA fragments-Transformation of bacteria-Antibiotic selectionPlasmids are autonomous replicating units capable of replicating in a limited (narrow host range) number of bacteria or broad (broad host range) range of different gram -ve bacteria by virtue of their replication regions. They also have well defined maps and many single restriction enzyme sites
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