HAU16008-02-2 - The Mechanism of Plant Transformation...

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1 The Mechanism of Plant Transformation Lecture 2 October 28, 2008 Core Concepts : Broad host range plasmid Chimeric genes Selectable marker genes Scorable marker genes Disarmed Agrobacterium strains Cointegrating vectors Binary vectors Multiplication/amplification of DNA molecules. Plasmid vectors -Narrow host range vectors -Broad host range vectors Cloning and Genetic selection -Cloning DNA fragments -Transformation of bacteria -Antibiotic selection Plasmids are autonomous replicating units capable of replicating in a limited (narrow host range) number of bacteria or broad (broad host range) range of different gram -ve bacteria by virtue of their replication regions. They also have well defined maps and many single restriction enzyme sites
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