HAU16008-02-3 - DNA AND CELL B IOLOGY Volume 2 1, Number 1...

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DNA AND CELL BIOLOGY Volume 21, Number 12, 2002 © Mary Ann Liebert, Inc. Pp. 963–977 Microparticle Bombardment as a Tool in Plant Science and Agricultural Biotechnology NIGEL J. TAYLOR and CLAUDE M. FAUQUET ABSTRACT Microparticle bombardment technology has evolved as a method for delivering exogenous nucleic acids into plant cells and is a commonly employed technique in plant science. Desired genetic material is precipitated onto micron-sized metal particles and placed within one of a variety of devices designed to accelerate these “microcarriers” to velocities required to penetrate the plant cell wall. In this manner, transgenes can be de- livered into the cell’s genome or plastome. Since the late 1980s microparticle bombardment has become a powerful tool for the study of gene expression and production of stably transformed tissues and whole trans- genic plants for experimental purposes and agricultural applications. This paper reviews development and application of the technology, including the protocols and mechanical systems employed as delivery systems, and the types of plant cells and culture systems employed to generate effective “targets” for receiving the in- coming genetic material. Current understanding of how the exogenous DNA becomes integrated into the plant’s native genetic background are assessed as are methods for improving the efficiency of this process. Pros and cons of particle bombardment technologies compared to alternative direct gene transfer methods and Agrobacterium based transformation systems are discussed. 963 INTRODUCTION T HE PRODUCTION of genetically transformed plant tissues and plants is now a central aspect of experimental plant science and a basic component of agricultural biotechnology. The worldwide area committed to transgenic crops has risen from 1 to 52.6 million hectares between in 1997 and in 2001 (James, 2001). The technology of microparticle bombardment has made an important contribution to these advances, facilitating the ge- netic transformation of a large range of plant species for fun- damental studies of gene regulation and the production of plants expressing agronomically useful traits. In a previous review of the subject, Luthra et al . (1997) documented 200 scientific pa- pers published between 1987 and 1995, which reported the use of particle bombardment technologies in plant science. Search- ing the databases in preparation for the present review article revealed that since 1995 more than 250 additional papers have been published in this area, illustrating the continued impor- tance of particle bombardment technology. In the following pages we describe particle bombardment, its relationship to other plant genetic transformation systems, and the impact it has had on plant science and biotechnology. TECHNOLOGIES FOR INSERTION OF
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HAU16008-02-3 - DNA AND CELL B IOLOGY Volume 2 1, Number 1...

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