blood lab

blood lab - Blood Laboratory Exercises Foreword It is well...

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Foreword: It is well known that many diseases may be transmitted by human blood or other body fluids. Although no human blood will be used in the following exercises, in the interest of promoting general safety practices, the set of “Universal (Standard) Precautions” intended to minimize the risk of contamination from handling human samples will be utilized throughout the entire laboratory session. It is essential that you do not deviate from these simple instructions. 1. Eating and drinking are strictly forbidden in the laboratory area. Wearing a lab coat is mandatory. 2. Protective disposable gloves must be worn at all times . Skin cuts or abrasions must be covered by a waterproof dressing prior to the donning of gloves. 3. Hands must be washed thoroughly following the removal of gloves, and prior to leaving the laboratory area. 4. All blood samples must be handled carefully – avoid spilling or splashing. 5. All used disposable materials (spectrophotometer cells, test tubes, gloves, etc.) and disposable sharp objects (Pasteur pipettes) must be placed in the cardboard box labeled with a “biohazard” symbol for disposal . 6. Non-disposable items such as the hemoglobinometer chamber and clip, the hemocytometer must be rinsed with water and must remain on the bench top. The difquik stained slide (WBC differentiation) must be returned to the central bench. In clinical practice, hematological information accumulated from a series of blood tests conducted on a small volume - even a single drop - of blood, can be of great diagnostic and prognostic value. Of the various kinds of simple blood tests available, three groups have been selected for this laboratory session: Part A - Measurement of Erythrocyte Fragility (p.2). Part B – Erythrocyte Sedimentation rate (p.4) Part C - Hemostatic Tests (p. 5) Part D - Blood Cell Indices (p. 7). Part E – Blood Group Typing (p.12) It is recommended that the entire group participate in each of the tests. To derive the most benefit from the following exercises, students are asked to review the information presented in class, as well as the appropriate chapters in the textbook dealing with the various constituents of blood, their functional characteristics, and the regulatory factors that determine their numbers or concentrations. NOTE : For reasons of safety, procedures for this lab have been modified. Some experimental procedures carried out on humans will be shown on video, while others will be performed using animal blood and using commercially-obtained simulated blood. Consequently, although instructions on drawing blood from human subjects are included so as to familiarize the student with the procedures, they should be disregarded for the actual experiments. Physiology
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This note was uploaded on 11/29/2010 for the course MATH 222 taught by Professor Karlpeterrussell during the Spring '08 term at McGill.

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blood lab - Blood Laboratory Exercises Foreword It is well...

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