200401M2-2

200401M2-2 - Chem 237 Midterm #2 from March 10, 2004 1....

Info iconThis preview shows pages 1–2. Sign up to view the full content.

View Full Document Right Arrow Icon

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon
This is the end of the preview. Sign up to access the rest of the document.

Unformatted text preview: Chem 237 Midterm #2 from March 10, 2004 1. Isolated β-subunits of HbA (normal hemoglobin) can be reassembled into β 4 tetramers using a specific set of conditions (you do not need to know these conditions to do the problem). These β 4 tetramers have a P 50 value of 6 torr and show no cooperativity. The isolated α- subunits of HbA have a pI of 7.4, and the β-subunits have a pI of 6.9. a. Assuming that you have purified HbA and have a method to selectively break the quaternary interactions between the α and β subunits, how might you separate the β- subunits from the α-subunits, using one of the separation techniques we have talked about in class? Be sure to include any pertinent details about the method you use (resin type, pH, salt concentration, etc). b. Draw an O 2 binding curve for the β 4 tetramers; be sure to label the axes properly, and include an Mb and Hb curve for reference. Why did you draw the curve the way you did?...
View Full Document

This note was uploaded on 12/02/2010 for the course CHEM 233 taught by Professor Daub during the Fall '09 term at Waterloo.

Page1 / 2

200401M2-2 - Chem 237 Midterm #2 from March 10, 2004 1....

This preview shows document pages 1 - 2. Sign up to view the full document.

View Full Document Right Arrow Icon
Ask a homework question - tutors are online