200401M2-3

200401M2-3 - h/ !-'/ Chem 37 M idterm# 2b M orchl O, Z O04...

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J,/ h/ !-'/ Chem 237 Midterm #2b Morch lO, ZO04 Nom, I.D.* Answer the questions on the paper provided. If you need more paper or clarification of a question, be sure to ask. *.*-- v 1. You have isolated and analyzed a peptide, with the following results. il the composition of the peptide: Ala 1 A*g 1 Ile 2 Tyr 1 Met 1 Arj i2- .--'/ A'J 0ln . t'. /, j'- Y 'r - -. i) ''' ei d n/'-. /l 3s iil the N'terminal amino acid is Arg '' f :s* iiil trypsin cleaves the peptide to generate a single amino acid and a pentapeptide i\ u',,, iy) chymotrypsin cleaves two tripeptides v) cNBr does not cleave at all. * L[,:f os rrr,4 t- prr,rutt.+{' vi), cAlloxypeptidase analysis of the intact peptrde was not clear, but you could tell that the two amino acids detected were Ala and Met. f6' \ a. What procedure would you use to generate the data in part G)? What are the conditions needed for this procedure? b. What conditions are needed to determine iii, iv and v? What does (vil telt you? c. Suggest a primary structure for the peptide. Use all the information above. d. How could you use Edman Degradation to simplifu this entire exercise? t I 'i\.jn N0 t\ltl+
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2. t',4C )') J \, (.*J,)\/\L' ,: \ Suppose, in true fairy-tale fashion, you were locked in a laboratory with purified enzyme and any equipment and reagents you needed., and were told to determine a Kn and V^r*values for the enzyme by dawn.
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200401M2-3 - h/ !-'/ Chem 37 M idterm# 2b M orchl O, Z O04...

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