Primary amino acid sequence problem Average amino acid residue MW~110g/mol CHEM 370 Determine the primary amino acid sequence of a peptide given the following information. 1. Composition of the peptide is Asp Cys Gly Lys Met Pro Ser Tyr 2. Molecular weight of the original peptide is ~1000 Da 3. Treatment of the original peptide with carboxypeptidase A and B generates NO free amino acid. 4. Treatment of the original peptide with Edman reagent yields a PTH-Ser 5. Chymotrypsin digestion of the original peptide yields a tetrapeptide and a pentapeptide 6. Treatment of the tetrapeptide from step 5 with Edman reagent yields a PTH-Cys 7. The pentapeptide from step 5 is found to have a free sulfhydryl group at the residue 2 amino acids from the C-terminus. 8. Trypsin digestion of the original peptide yields a tripeptide and a hexapeptide 9. The tripeptide from the step 8 contains an amino acid with an acidic R-group 10. Cyanogen bromide digestion of the hexapeptide from step 8 yields a dipeptide and a tetrapeptide
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