BIO311C_F10_Chapter_16

BIO311C_F10_Chapter_16 - DNA structure and replication...

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Unformatted text preview: DNA structure and replication Chapter 16 (pages 305-316) Relevant parts What we will cover • Experiments done to show DNA was the genetic material • Review DNA structure • Different types of replication • Experiments to show semiconservative replication • Mechanism of replication • Enzymes in replication Evidence That DNA Can Transform Bacteria • Frederick Griffith was studying Streptococcus pneumoniae – A bacterium that causes pneumonia in mammals • He worked with two strains of the bacterium – A pathogenic strain (Smooth) and a nonpathogenic strain (Rough) 1928 Griffith experiment Fig. 6.3 b Genetics: from genes to genomes (Hartwell) • Subsequent experiments suggested that the causative agent: DNA • Transformation: – The ability of foreign DNA to change the genetic characteristics of an organism • Viruses that infect bacteria – bacteriophages (bacteria eaters) re 16.3 Phage head Tail Tail fiber DNA Bacterial cell 100 nm Fig. 16-4-3 EXPERIMENT Phage DNA Bacterial cell Radioactive protein Radioactive DNA Batch 1: radioactive sulfur ( 35 S) Batch 2: radioactive phosphorus ( 32 P) Empty protein shell Phage DNA Centrifuge Centrifuge Pellet Pellet (bacterial cells and contents) Radioactivity (phage protein) in liquid Radioactivity (phage DNA) in pellet http://highered.mcgraw-hill.com/olc/dl/120076/bio21.swf Erwin Chargaff fyi Chargaff’s rule The amount of Adenine equals the amount of Thymine The amount of Guanine equals the amount of Cytosine The Watson-Crick Model: DNA is a double helix • 1951 – James Watson learns about x-ray diffraction pattern projected by DNA • Knowledge of the chemical structure of nucleotides • Erwin Chargaff’s experiments demonstrate that ratio of A and T are 1:1, and G and C are 1:1 1953 – James Watson and Francis crick propose their double helix model of DNA structure Fig. 16-7a Hydrogen bond 3 ′ end 5 ′ end 3.4 nm 0.34 nm 3 ′ end 5 ′ end (b) Partial chemical structure (a) Key features of DNA structure 1 nm DNA replication...
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This note was uploaded on 12/04/2010 for the course BIO 311 C taught by Professor Mcclelland during the Fall '09 term at University of Texas.

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BIO311C_F10_Chapter_16 - DNA structure and replication...

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