BIO311C_F10_Chapter_17_part1

BIO311C_F10_Chapter_17_part1 - Gene expression Process by...

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Unformatted text preview: Gene expression Process by which DNA directs synthesis of proteins (or just RNA) Involves: Transcription Translation Central dogma of modern biology Transcription and RNA processing Pages 331-336 Transcription DNA directed synthesis of RNA Comparing DNA and RNA What we will cover Where does transcription occur in a eukaryotic as against a prokaryotic cell? Process of transcription Pre-mRNA processing in eukaryotes Exons vs. introns Fig. 17-3b-3 TRANSCRIPTION Nuclear envelope DNA Pre-mRNA RNA PROCESSING mRNA TRANSLATION Ribosome Polypeptide Eukaryotes Transcription and Translation occur in separate compartments RNA polymerase II forms pre mRNA (primary transcript) Processed (modified) to mRNA Prokaryotes Prokaryotic cell. In a cell lacking a nucleus, mRNA produced by transcription is immediately translated without additional processing. (a) TRANSLATION TRANSCRIPTION DNA mRNA Ribosome Polypeptide Prokaryotes Transcription and translation are coupled RNA made on transcription is mRNA RNA polymerase (RNAP) - transcription Some nomenclatures Promoters: DNA sequences near the beginning of genes that signal RNA polymerase where to begin transcription RNA polymerase binds the promoter Terminators: Sequences in the RNA product that tells RNA polymerase where to stop (encoded by the DNA) Transcription unit: stretch of DNA transcribed into RNA Some more nomenclature convention (coding strand) (sense strand) (non-coding strand) (anti-sense strand) RNAP 5...
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This note was uploaded on 12/04/2010 for the course BIO 311 C taught by Professor Mcclelland during the Fall '09 term at University of Texas at Austin.

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BIO311C_F10_Chapter_17_part1 - Gene expression Process by...

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