Lecture 4 - Lecture 4 Overview Mismatch Repair What is...

Info iconThis preview shows pages 1–5. Sign up to view the full content.

View Full Document Right Arrow Icon
Lecture 4 Overview Mismatch Repair What is mismatch repair How was mismatch repair discovered How does it work What damage does it repair
Background image of page 1

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon
DNA mismatch repair (MMR): What is it? • corrects mismatches generated during DNA replication that escape proofreading • conserved process from E. coli to Yeast to Humans • post-replicative mismatch repair: excision of DNA segment with incorrect base, followed by resynthesis of DNA
Background image of page 2
Importance of E. coli Mismatch repair • enhances fidelity of DNA synthesis by 100 to 1000-fold: e.g. replication fidelity is one mistake in 10 6 to 10 8 incorporated bases wo. MMR, but increases w. MMR to one mistake in 10 9 to 10 10 incorporated bases Disruption of MMR function in E. coli: -increased rate of genome-wide point mutations -increased mutation rate provides genetic diversity in cells – can ultimately improve ‘fitness’ of microbial populations e.g. ability for emergence of resistant strains
Background image of page 3

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon
Importance of Mammalian MMR loss of MMR activity results in ‘mutator’ phenotype • increase in genome-wide point mutations • problems in exact replication of repetitive DNA sequences loss of MMR activity results in ‘microsatellite instability’
Background image of page 4
Image of page 5
This is the end of the preview. Sign up to access the rest of the document.

This note was uploaded on 12/03/2010 for the course PHAMACOLOG pcl470 taught by Professor Arnot during the Fall '10 term at University of Toronto- Toronto.

Page1 / 13

Lecture 4 - Lecture 4 Overview Mismatch Repair What is...

This preview shows document pages 1 - 5. Sign up to view the full document.

View Full Document Right Arrow Icon
Ask a homework question - tutors are online