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CSB349L20-review - CSB349 Lecture 20 November 29th k now...

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CSB349 Lecture 20 November 29 th know general concepts and techniques. 10 SA. These are really easy. Remember to be clear and succinct. Point form is acceptable as long as they are clearly written sentences. Blue or black ink only, no pencil.
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CSB349 Lecture 20 November 29 th We looked at two main subtopics for gene silencing: DNA methylation –role of DNA methyltransfereases, maintenance vs. de novo methylation, ways to study it (bisulfite sequencing and a subsequent step to assay the gene either PCR or primers designed to detect methylated sequences). All genome: high throughput techniques like microarrays in addition to bisulfite sequencing (methylomics). CpG islands and their link to cancer. How DNA methylation, in the promoter (where it’s most relevant w/ respect to regulation of gene expression…dictates whether or not the transcription machinery is properly working). DNA methylation matters where proteins recognize it and bind to it (meCPII). Causes histone deacetylases That are the key factors that translate methylated DNA to gene silencing. How myc (TF), can be recruited to gene promoter and helps recruit DNAmethyltransferase Dnmt3a to the promoter of P21. Once the proteins are binding and recruiting to promoter, they influence gene expression. DNA methylation is also used in monoallelic expression. Detect which allele is being expressed. Design specific transgenes that have regulatory and reporter sequences. Another
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