CSB349L2 - CSB349 Lecture 2 Welcome to the genome Repeats...

Info icon This preview shows pages 1–3. Sign up to view the full content.

View Full Document Right Arrow Icon
CSB349 Lecture 2 September 15 th , 2010 Welcome to the genome: Repeats Prof. Alan Moses email: [email protected] Wednesday lectures are usually an hour, not the full two hours. Tutorials start this week. Mine is 1701? w/ James in SS1080. The majority of the items discussed in class will have been discussed previously however, this class will go over the experiments which lead to the discoveries that we discuss. Prof. Moses wants to give a tour of what is DNA and of gene regulation at the level of proteins. Genome (transcriptional units, repetitive DNA, non-repetitive and non-coding DNA, reversible gene silencing, modular regulatory elements) Transcriptome Proteome (Post-translationally modified and unmodified proteins …e.g. enzymatically cleaved; covalently modified). Prof. Moses insisted the use of fig 7-5 from Alberts. He is going to discuss the genome and transcriptional control and then protein activity control. For the purposes of understanding genes, a simple plastic model is not informative or accurate. The question to be discussed is what is really in the human genome? The human genome is probably one of the few genomes that we have a complete sequence for that is represented of a species that wasn’t selected for a particular biological property. There might be reasons why it is a representative of a genome that was created by evolution rather than chosen by us. The era where all of us will have our genome sequenced is arriving soon. The human genome is 3.08 gigabases but only 2.85 gigabases was sequenced. Missing 200 million bases and most of that is heterchromatin, telomeres, and repetitive DNA. There was a 10^-5 error rate (which is a mistake every 1 in 100,000). The human genome is largely composed of non-transposable elements (55%), protein coding genes only make up a small percent..most is non-coding. A large portion (45%) is composed of repetitive sequences. Why does the genome have so much space devoted to things which are not genes. Most of our genome is not actually human genes. Only 20,000 -25,000 is actually human protein-coding genes. Repetitive sequences. There are many types of repetitive DNA. Mechanistic classification. 1. Tandemly repeated sequences (arranged in tandem on the genome, next to each other, in big blocks in genome sequence) a. Satellite DNA (largest pieces of tandem DNA. Classic type is centromeres: huge pieces of heterochromatic DNA made of simple repeating units. 10, 50, 100 bps repeated over and over)
Image of page 1

Info icon This preview has intentionally blurred sections. Sign up to view the full version.

View Full Document Right Arrow Icon
CSB349 Lecture 2 September 15 th , 2010 b. Minisatellite DNA (smaller, like telomeres. Made of small repeating units 10-12 bps repeated
Image of page 2
Image of page 3
This is the end of the preview. Sign up to access the rest of the document.

{[ snackBarMessage ]}

What students are saying

  • Left Quote Icon

    As a current student on this bumpy collegiate pathway, I stumbled upon Course Hero, where I can find study resources for nearly all my courses, get online help from tutors 24/7, and even share my old projects, papers, and lecture notes with other students.

    Student Picture

    Kiran Temple University Fox School of Business ‘17, Course Hero Intern

  • Left Quote Icon

    I cannot even describe how much Course Hero helped me this summer. It’s truly become something I can always rely on and help me. In the end, I was not only able to survive summer classes, but I was able to thrive thanks to Course Hero.

    Student Picture

    Dana University of Pennsylvania ‘17, Course Hero Intern

  • Left Quote Icon

    The ability to access any university’s resources through Course Hero proved invaluable in my case. I was behind on Tulane coursework and actually used UCLA’s materials to help me move forward and get everything together on time.

    Student Picture

    Jill Tulane University ‘16, Course Hero Intern