CSB349L4 - CSB349H1 Lecture 4 September 22nd Welcome to the...

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CSB349H1 Lecture 4 September 22 nd Welcome to the genome III: Genome structure. Repetitive elements, transposons, genes. What is the structural conformation of the genome, how does it sit inside the cell. What are the important structural features? Nucleus is heterogeneous. There are multiple different types of conformation in which you’ll find the genome in the nucleus. We’ll look at the heterochromatic regions and euchromatic regions. The majority of the DNA is in a euchromatic state. How are these different types of DNA organized in the nucleus. There are proteins inside it that form it in a specific conformation. Nuclear matrix hold the genome in a particular conformation. The nucleolus can move around, the network holding the genome is very flexible and dynamic. The chromosomes in the nucleus are not like spaghetti in a bowl, there is some organization to where the chromosomes are located in the nucleus. FSH image: specific probes have been labelled to the chromosomes (set for chromosome 7 and 8...diploid). One of the copies of chromosome 7 and 8 are close, the other copies are separated. They are extended and snaking without the nucleus (?????maybe???). Does their location mean anything? Chromosome territories: They tend to maintain a spatially defined volume w/in the nucleus that is spherical in shape. The chromosomes can be adjacent to one another, the positions are probabilistic (among a population of cells). The material of the nucleus is not definitely in a particular location...they generally tend to be in those locations though, on average. Is the specific organization important? Heterchromatic regions are clumped together rather than euchromoatic? Genes that are going to have different levels of expression in a developing organism, those genes might want to be located near one another in the nucleus. *************** Experiments to study the organization within the nucleus. Histone (proteins accosiated w. The
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