CSB349L8 - CSB439 Lecture 8 October 6th In the past...

Info iconThis preview shows pages 1–3. Sign up to view the full content.

View Full Document Right Arrow Icon
CSB439 Lecture 8 October 6 th In the past lectures, we’ve discussed transcriptional control (chromatin regulation. ...), where and how much a gene can be transcribed. We’re now going to look on the mechanisms of transcription. Moved to ON chromatin, the green and blue bubbles are SSTF, interacting w/ the mediator (which will be discussed today). The DNA that they’re bound to, corresponds to an enhancer. TFbinding sites nearby promoter. TF, enhancers, stuff, sit down on promoter and make transcripts. The general point is that the entire complex (w/o the mediator), is called the pre-initiation complex (PIC), the formation of this complex is the rate-limiting step in transcription initiation= if you form this complex, you will get some transcripts made. All other things help, stabilize, and form the PIC. PIC large multi-subunit mediator complex. You can reconstitute transcription in vitro using the components of this complex. Possible by assembling the components together (TFII, RNA polymerase. .). These componenets critical and sufficient. TFIIA, B, C, etc. Are general (basic), TF different from SSTF.
Background image of page 1

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full Document Right Arrow Icon
CSB439 Lecture 8 October 6 th General Transcription factors: highly conserved. Don’t have stereotypical structural domains (DNA binding, activation). They form the basic structure of the PIC. They assemble into a pile of proteins w/ the RNA polymerase. They melt the duplex (separate the double helix). PIC assembly is sequential, as determined by DNase1 footprinting, EMSA, and ChIP. We are only considering transcription by RNA polymerase II (most protein coding genes, snRNA,
Background image of page 2
Image of page 3
This is the end of the preview. Sign up to access the rest of the document.

{[ snackBarMessage ]}

Page1 / 11

CSB349L8 - CSB439 Lecture 8 October 6th In the past...

This preview shows document pages 1 - 3. Sign up to view the full document.

View Full Document Right Arrow Icon
Ask a homework question - tutors are online