CSB349L9 - CSB349 Lecture 9 October 13th RNA Pol Might be...

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CSB349 Lecture 9 October 13 th RNA Pol. Might be sitting on the promoter and ignition might be happening and short transcripts may be made by the PIC itself! Pol leaving the promoter and making short transcripts (initiation) After the initiation phase of transcription, there’s the elongation phase (creation of very large transcripts). Pol leaves promoter completely and begins to make a long RNA transcript. From ignition to elongation phase, all these things are going on at the same time. At initiation the RNA pol C term domain is phosphorylated. Recruit capping factors which will modify 5’ end of transcript. Short nascent transcript being formed. All done in initiation phase. As the complex transitions to elongation, you have a recruitment of the splicing factors (capping factors have moved onto the cap). The complex of proteins
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CSB349 Lecture 9 October 13 th that mediate the splicing is recruited by the RNA pol as the transcript is being made. In final picture, in productive elongation, we recruite polyadenylation and cleavage factors. As pol complex moves from one phase to another, it recruits different factors. At this transition phase, most of the general TF ( TFIID)are lost, move away from the promoter and a new set of TF replace them (TFIIS and Spt5) which are elongation factors. C- terminal domain mediates 5’ capping, splicing, and polyA and cleavage. These different events are mediated by these three events independedntly..post-translational modification of the C-term changes over the stages of initiation to elongation. The serine is phosphorylated by TFIIH and this helps recruit capping machinery. During elongation, the phosphates are removed and a new set of phosphorylation is added b P-TEFb (also on S2) which leads to change in affinity for different co-factors, now splicing and polyA..It’s all just phosphorylation of S2????
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CSB349 Lecture 9 October 13 th RNA PolIII and I don’t have the cap that RNA pol II has. Many of the Pol II transcripts are prevents degredation enzymes, helps transport RNA to cytoplasm and helps it recognize the 5’. We’re not going to talk about translation.
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