handout10-DNA - Bio 311C Buskirk What data contributed to...

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Bio 311C, Buskirk What data contributed to knowledge of DNA structure? (Double Helix) Knowledge from organic chemistry; composition of a nucleotide : a 5-carbon sugar (deoxyribose), a phosphate, and nitrogenous base (a double ring or single ring of N, C and H) purines (double ring) A=adenine, G=guanine pyrimidines (single ring) T=thymine, C=cytosine [Learn the 1’,3’ (3 prime), 5’ (5 prime) notation] from Linus Pauling : by 1950, Chargaff's Rules: [A=T C=G ] •the idea to build a 3-dimensional model the proportion of different nitrogenous bases •compare predicted model with lab measures in DNA is species-specific. The amount of •in 1951, found alpha helix form of proteins adenine equals the amount of thymine, and the % cytosine equals % guanine, e.g., in humans, from M. Wilkins' lab: approximately 30% A, 30% T, 20% C, 20% G •Wilkins prepared uniformly aligned DNA fibers, best samples for crystalline DNA From biochemistry laboratories: •Rosalind Franklin performed the best, most Purified DNA, when mildly heated, changes form careful X-ray crystallography of DNA fibers.
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  • Spring '08
  • Satasivian
  • Biology, DNA, DNA nucleotide components

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