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Unformatted text preview: 514 EXAMPLE: Ryanodine Recepter (RyR) [ryrpaper.pdf] PDB entry 2K2F. BACKGROUND: many cellular responses initiated by cytosolic Ca 2 + : (muscle contraction, neurotransmitter release, secretion, gene transcription. .) release channels: amplify signal by releasing more Ca 2 + This results in a sudden increase of calcium (Calcium spark). NOTE: A number of sicknesses related to incorrect regulation of calcium spark. PROBLEM: membrane bound proteins difcult to study in situ . SOLUTION: Introduce marker molecules whose ourescence depends on calcium concentration. can observe calcium spark behaviour in cells in the presence of drugs (e.g. caffeine increases sensitivity ( muscles more twitchy!)) 515...
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