9 - VertPhysNotes9/9 11/09/201012:47:00

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Vert Phys Notes 9/9 11/09/2010 12:47:00 Patch clamp = method of recording Na+ channels Fire polish pipet, on avg get no channel, sometimes 1 or 2, or more o Need to make tight seal with tip of poet and membrane Suck in with mouth, pulls membrane up through glass Tight bond occurs, release suction, doesn’t fall off Further suction can rupture membrane Allows you to measure current Whole-cell recording Inside out recording and outside out recordings Traces from single channel do not look like mult channels o Behavior different, whole =looking at avg o Occur in open and close states When open = activation gate opens, Na+ in  When closed = inactivation gate plugs from inside o 2 gates open by depolarization active and inactivated gates o Single channel sampled many times, same as many channels sampled Behave probabilistically 
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This note was uploaded on 12/06/2010 for the course PHYS 365R taught by Professor Pollak/zakon during the Fall '10 term at University of Texas.

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9 - VertPhysNotes9/9 11/09/201012:47:00

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