Lec 7 - Regulation of eukaryotic gene expression(pp 282-290 Regulatory sequences in protein-coding genes Genes that are transcribed at high

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Regulation of eukaryotic gene expression (pp. 282-290)
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Regulatory sequences in protein-coding genes Genes that are transcribed at high levels (have strong promoters) have a TATA box starting 35 bp upstream of the start site. The TATA box functions similarly to an E. coli promoter, positioning RNA polymerase for transcription initiation.
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Alternatives to the TATA box • Some genes have an initiator element which includes a C at the -1 position and an A at the +1 position. No good consensus sequence has been defined. • Transcription of some genes initiates at multiple sites within a 20-200 bp region. These genes lack a TATA box or an initiator element, but contain a CG-rich stretch of 20- 50 bp (called a CpG island) within ~100 bp of the start-site region.
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Promoter-proximal elements • In this course, the term promoter will be used for the TATA box or other sequence that recruits RNA polymerase to the transcription start site. • Other sequences near the promoter (within 100-200 bp) can regulate transcription. These are called promoter-proximal elements. • Promoter-proximal elements can be cell type specific.
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Linker-scanning mutations can be used to find promoter-proximal elements This experimental approach is good for detailed analysis of a short stretch of DNA.
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This note was uploaded on 12/07/2010 for the course BIO BIOL 200 taught by Professor Frogatto during the Fall '10 term at McGill.

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Lec 7 - Regulation of eukaryotic gene expression(pp 282-290 Regulatory sequences in protein-coding genes Genes that are transcribed at high

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