lec 9 - Quiz 2 No class on Friday, October 1 Lectures up to...

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Unformatted text preview: Quiz 2 No class on Friday, October 1 Lectures up to Monday, September 27 inclusive Open book Accessible from 06:00 Friday to 21:00 Sunday Post-transcriptional steps of gene expression Overview of eukaryotic mRNA processing Addition of the 5 cap structure 7-methylguanosine is added to the 5 end of the nascent mRNA when it is 25-30 nt long. This is catalyzed by a dimeric capping enzyme, that associates with the CTD of RNA Pol II. One subunit removes the -phosphate from the 5 end of the RNA, and the other transfers GMP from GTP to the 5 diphosphate of the nascent transcript. Separate enzymes then transfer methyl groups to the N 7 position of the guanine . RNA binding domains Many were discovered in hnRNPs, proteins that associate with pre-mRNAs. RNA recognition motif (RRM): the most common RNA binding domain. 80 amino acids, folds into 4-stranded sheet flanked by 2 -helices. Contains RNP1 and RNP2 motifs that contact the phosphates of RNA. Other RNA binding motifs...
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This note was uploaded on 12/07/2010 for the course BIO BIOL 200 taught by Professor Frogatto during the Fall '10 term at McGill.

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lec 9 - Quiz 2 No class on Friday, October 1 Lectures up to...

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