lec 9 - Quiz 2 No class on Friday October 1 Lectures up to...

Info icon This preview shows pages 1–8. Sign up to view the full content.

View Full Document Right Arrow Icon
Quiz 2 No class on Friday, October 1 Lectures up to Monday, September 27 inclusive Open book Accessible from 06:00 Friday to 21:00 Sunday
Image of page 1

Info icon This preview has intentionally blurred sections. Sign up to view the full version.

View Full Document Right Arrow Icon
Post-transcriptional steps of gene expression
Image of page 2
Overview of eukaryotic mRNA processing
Image of page 3

Info icon This preview has intentionally blurred sections. Sign up to view the full version.

View Full Document Right Arrow Icon
Addition of the 5’ cap structure 7-methylguanosine is added to the 5’ end of the nascent mRNA when it is 25-30 nt long. This is catalyzed by a dimeric capping enzyme, that associates with the CTD of RNA Pol II. One subunit removes the γ -phosphate from the 5’ end of the RNA, and the other transfers GMP from GTP to the 5’ diphosphate of the nascent transcript. Separate enzymes then transfer methyl groups to the N 7 position of the guanine .
Image of page 4
RNA binding domains Many were discovered in hnRNPs, proteins that associate with pre-mRNAs. RNA recognition motif (RRM): the most common RNA binding domain. 80 amino acids, folds into 4-stranded β sheet flanked by 2 α -helices. Contains RNP1 and RNP2 motifs that contact the phosphates of RNA.
Image of page 5

Info icon This preview has intentionally blurred sections. Sign up to view the full version.

View Full Document Right Arrow Icon
Image of page 6
Other RNA binding motifs
Image of page 7

Info icon This preview has intentionally blurred sections. Sign up to view the full version.

View Full Document Right Arrow Icon
Image of page 8
This is the end of the preview. Sign up to access the rest of the document.

{[ snackBarMessage ]}

What students are saying

  • Left Quote Icon

    As a current student on this bumpy collegiate pathway, I stumbled upon Course Hero, where I can find study resources for nearly all my courses, get online help from tutors 24/7, and even share my old projects, papers, and lecture notes with other students.

    Student Picture

    Kiran Temple University Fox School of Business ‘17, Course Hero Intern

  • Left Quote Icon

    I cannot even describe how much Course Hero helped me this summer. It’s truly become something I can always rely on and help me. In the end, I was not only able to survive summer classes, but I was able to thrive thanks to Course Hero.

    Student Picture

    Dana University of Pennsylvania ‘17, Course Hero Intern

  • Left Quote Icon

    The ability to access any university’s resources through Course Hero proved invaluable in my case. I was behind on Tulane coursework and actually used UCLA’s materials to help me move forward and get everything together on time.

    Student Picture

    Jill Tulane University ‘16, Course Hero Intern