lec 16 - Lecture 16: DNA replication General principle:...

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Lecture 16: DNA replication
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Model 1 Red: parental strands Blue: daughter strands General principle: conservative or semi-conservative? Model 2
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Meselston-Stahl experiment: grow cells in medium with 15 N -> all DNA labeled wash 15 N and grow cells in “cold medium” ( 14 N ) -> new synthesized DNA = unlabeled separation of “old” ( heavy ) and “new” ( light ) DNA by centrifugation
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Meselston-Stahl experiment:
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Meselston-Stahl experiment: only parental = H “hybrid” old/new H L ½ “hybrid” H L ½ new LL
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DNA polymerases require a primer to initiate replication Quality control: the polymerase can add a new nucleotide only if the previous pair is OK = Proofreading RNA synthesis: 1 error in 10 4 DNA synthesis: 1 error in 10 9 !!
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What is the first step required to allow DNA synthesis?
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Replication fork: Duplex DNA is unwound
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Duplex unwinding: topoisomerase toposisomerase toposisomerase toposisomerase torsion force flip (one turn) cut repair
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Replication fork: formation of daughter strands Short RNA primer
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This note was uploaded on 12/07/2010 for the course BIO BIOL 200 taught by Professor Frogatto during the Fall '10 term at McGill.

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lec 16 - Lecture 16: DNA replication General principle:...

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