lec 26 - > reaction Products cause reopening Enzymes...

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Lecture 26: Protein function (Ch. 3; p . 73-83 )
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Ligand-binding: 1) selectivity hydrophobic amino acids Polypeptide A: backbone polypetide B (general shape of surface) Ligand-binding: 1) affinity (how strong they bind) affinity constant = Kd
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Molecular complementarity: antibodies Antibody structure CDR = complementarity- determining region
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Molecular complementarity: antibodies contact between antibody and antigen “fingers” (6 variable loops) region of antigen recognized by one specific antibody = epitope ! epitope is not necessarily a linear sequence!
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Enzymes are efficient and specific catalysts A+B A*B* C+D transition state
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Enzymes: Protein Kinase A Ser or Thr in polypeptide + ATP P-Ser (P-Thr) + ADP
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Enzymes: Protein Kinase A ATP trapped by the glycine lid Charged residues in pocket stabilize phosphate groups Specific sequence of peptide (Arg-Arg-X- Ser -Y) bound to hollow groove
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Enzymes: Protein Kinase A Open position allows ATP and substrate to enter Binding induces change in conformation -> closed Peptide and ATP brought in close proximity
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Unformatted text preview: -> reaction Products cause reopening Enzymes: Protein Kinase A Catalysis: bringing reactants together + participation of enzyme residues in formation of intermediate state -> reduces activation energy Enzymes: Protein Kinase A Specific sequence of peptide (Arg-Arg-X-Ser-Y) Enzymatic reactions are highly specific: Only some specific peptide sequences can bind any aa hydrophobic Evolution of multifunctional enzymes Enzymatic cascades can be organized around scaffold proteins membrane receptor MAP-kinase-kinase-kinase P P phosphorylation of several targets MAP-kinase-kinase MAP-kinase Example: MAP-kinase pathway Molecular motors: linear motors/rotary motors Molecular motors moving along DNA/RNA Molecular motors moving along cytoskeletal filaments Molecular motors: ATP synthase Demonstration of rotation of the ATP synthase (F1)-subunits linked to support fluorescent actin filament linked to -subunit + ATP (! Inverted reaction, ATP hydrolysis)...
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