Problem Set 7 Q&A - Problem Set 7 Cellular...

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Problem Set 7 Cellular Neurobiology Fall 2008 1. The following question is from Reyes et al. Target-cell-specific facilitation and depression in neocortical circuits. Nat Neurosci (1998) vol. 1 (4) pp. 279-285. a) Describe the set-up of the experiment in this figure 3 briefly (what cells, what technique, and so on). In developing rodent’s brain slices, the authors recorded simultaneously from layer 2/3 pyramidal cells and two classes of interneuron cells- bitufted cells and multipolar cells, using whole-cell recordings. Current clamp experiments were performed. Blue = pyramidal cells, red = multipolar, green = bitufted b) How did the authors show that the synapse was glutamatergic? How did they show that the interneurons were GABAergic? Authors used AMPA receptor antagonists and NMDA receptor antagonists (30 µM CNQX and 50 µM APV, respectively) and showed that they blocked the excitatory transmission . They then used antagonists of GABA receptors (20 µM of bicuculline), which blocked the inhibitory transmission. c) What do the data in figure 3 tell you? The data in figure 3 indicate that a single neuron can express both facilitating (P M) and depressing (P B) synapses. d) Given what you have learned in the class thus far, what is the mechanism of the above forms of plasticity? (pre- or post-, molecular explanations, etc) Both were pre-synaptic events. Depression resulted from exhaustion of SV’s from the readily releasable pool and this is observed in synapses where
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there is high probability of release. Facilitation resulted from increase in local Ca 2+ concentration. 2. The following question is from Professor Scanziani’s lecture and Pouille et al. Routing of spike series by dynamic circuits in the hippocampus. Nature (2004) vol. 429 (6993) pp. 717-723.
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