MCB354FA08_ExamIIAKEY (1) - True-False(16 points total 4...

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1 True-False (16 points total, 4 points each) Select True or False for the following statements. 1. The raw data that one obtains from a single x-ray diffraction study will allow the frequency, amplitude and phase to be determined for all of the waves scattered from the protein crystal. A. True B. False 2. According to Bragg’s Law, there will be constructive interference between two rays, striking two different parallel planes of a lattice, if the additional distance traveled by the more deeply penetrating wave is an integral multiple of the wavelength, λ . A. True B. False 3. The unit cell of a crystal is always at least as small as the smallest asymmetric unit. A. True B. False 4. The hanging drop method for crystallization of proteins is a “diffusion method”, in which the protein and the precipitant concentrations slowly change, which may create conditions favorable for protein crystal formation and growth. A. True B. False
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2 Multiple Choice (88 points total, 4 points each) For each question below, select one correct answer (A, B, C, D, or E) and fill in the bubble on the scantron. 5. Which of the following statements concerning the process of spontaneous folding of proteins is false ? A. It may be an essentially random process. B. It may be defective in some human diseases. C. It may involve a gradually decreasing range of conformational species. D. It may involve initial formation of a highly compact state. E. It may involve initial formation of local secondary structure. 6. Protein S will fold into its native conformation only when protein Q is also present in the 7. Experiments on denaturation and renaturation after the reduction and reoxidation of the —S—S— bonds in the enzyme ribonuclease (RNase) have shown that:
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